Table 3.
Validation of mixed SSU rRNA using Canadian and Swedish isolates.
| Sample origin | Number samples with mixed sequences | Number (%) samples with mixed species/genotypes correctly identified on at least one strand | Number (%) samples with mixed species/genotypes incorrectly identified on at least one strand | Number (%) samples identified as a single species/genotype | Number (%) samples with one species/genotype correctly identified | Number (%) samples with one species/genotype incorrectly identified | Number (%) samples with one species/genotype correctly identified but second species/genotype further resolved with manual analysis |
|---|---|---|---|---|---|---|---|
| Canada: | |||||||
| Human | 55 | 38 (69.1%) | 0 (0%) | 17 (30.9%) | 13 (76.5%) | 0 (0%) | 4 (23.5%) |
| Animala | 107 | 94 (87.9%) | 0 (0%) | 13 (12.1%) | 13 (100%) | 0 (0%) | 0 (0%) |
| Water | 18b | 10 (55.6%) | 1 (5.6%) | 7 (38.9%) | 7 (100%) | 0 (0%) | 0 (0%) |
| Sweden: | |||||||
| Animalc | 20 | 12 (60.0%) | 1 (5.0%) | 7 (35.0%) | 5 (71.4%) | 0 (0%) | 2 (28.6%) |
| Total: | 200 | 154 (77.0%) | 2 (1.0%) | 44 (22.0%) | 38 (86.4%) | 0 (0%) | 6 (13.6%) |
a
Animal sample sources from 95 cattle, 2 dogs, 6 cats, 1 pig, 3 snakes.
b
1 sample was not typable either by CryptoGenotyper or manually so have been removed from this total.
c
Animal sample sources from 7 cattle, 10 squirrels, 2 chital deer, 1 sheep.