Figure 2.

Chemical structure and dose response of nitazoxanide against nsp14. (A) Chemical structures of nitazoxanide (1) and tizoxanide (2). (B) Dose–response testing of nitazoxanide and tizoxanide against nsp14 in a primary screening assay. Compounds were tested at 10 concentrations, 1:2 from 100 µM (top concentration). The IC₅₀ of nitazoxanide was 9.7 µM (6.3-15.1 µM; n = 4), and the IC₅₀ of tizoxanide was 54.8 µM (27.3–110.0 µM; n = 4). (C) Dose–response testing of nitazoxanide against nsp14 in an orthogonal radiometric assay with a representative phosphoimage. Data are shown as mean ± SD.