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. 2021 Feb 7;296:100388. doi: 10.1016/j.jbc.2021.100388

Figure 5.

Figure 5

Nitric oxide inhibits ATR-dependent DDR signaling in INS 832/13 cells. INS 832/13 cells were treated with 5 mM hydroxyurea for 2 h in the presence or absence of 400 μM DPTA/NO and 400 μM cPTIO (A), or hydroxyurea was added after a 24-h incubation with 10 U/ml IL-1β in the presence or absence of 2 mM NMMA (B). Cells were then stained for phospho-Chk1 (green), γH2AX formation (red), and nuclei (DAPI, blue) and imaged via Nikon Eclipse 90i confocal microscope (60× with 2× field zoom). Results are representative of three independent experiments. ATR, ataxia–telangiectasia and Rad3-related protein; cPTIO, 2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazolyl-1-oxy-3-oxide; DDR, DNA damage response; DPTA/NO, (Z)-1-[N-(3-aminopropyl)-N-(3-ammoniopropyl)amino]diazen-1-ium-1,2-diolate; NMMA, NG-monomethyl-l-arginine; DAPI, 4′,6-diamidino-2-phenylindole.