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. 2021 Feb 7;296:100388. doi: 10.1016/j.jbc.2021.100388

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Extracellular flux analysis of INS 832/13 cells and MEF. The oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of INS 832/13 cells (A and B) and MEF (C and D) were measured over a 2-h incubation. The time of DPTA/NO (400 μM), rotenone (1 μM), antimycin A (10 nM), or FCCP (5 μM) addition is indicated by the black arrows. Results are the average ± SEM of three independent experiments. DPTA/NO, (Z)-1-[N-(3-aminopropyl)-N-(3-ammoniopropyl)amino]diazen-1-ium-1,2-diolate; FCCP, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; MEF, mouse embryonic fibroblast.