Figure 7.

AKAP1 is feedback downregulated under obese conditions. a) The mRNA expression levels of AKAP1 were evaluated in the human adipose tissue based on public microarray expression datasets (GSE92405, GSE59034, GSE77962). Cont, never‐obese control; bBS, before bariatric surgery; aBS, after bariatric surgery; bWL, before weight loss (at study start); aWL, after weight loss period. b) The mRNA expression levels of AKAP1 were evaluated in the human liver and muscle based on public microarray expression datasets (GSE48452, GSE135066). c,d) Relative mRNA expression (left) and Western blotting analysis (right) of AKAP1 expression in adipose tissues from ob/ob and db/db mice (n = 3 mice per group). e,f) Relative mRNA expression (left) and Western blotting analysis (right) of AKAP1 expression in liver and muscle from ob/ob and db/db mice (n = 3 mice per group). g,h) Relative mRNA expression (left) and Western blotting analysis (right) of AKAP1 expression in adipose tissues from mice after HFD feeding for 6 and 24 weeks (n = 3 mice per group). i) Relative mRNA expression (left) and Western blotting analysis (right) of AKAP1 in mouse brown adipocytes pretreated with palmitate for 24 h. Data were from three independent experiments. j) Relative mRNA expression (left) and Western blotting analysis (right) of AKAP1 in human adipocytes pretreated with palmitate for 24 h. Data were from at least three independent experiments. Data were expressed as mean ± SEM. Student's t‐test was used in (a) (GSE92405, GSE77962) and (c–h). One‐way ANOVA with Bonferroni's post hoc test was used in (a) (GSE59034), (b), (i), and (j). *p < 0.05, **p < 0.01.