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. 2021 Jan 27;8(6):2004032. doi: 10.1002/advs.202004032

Figure 6.

Figure 6

CD146 interacts with ANGPTL2. A) Analysis of CD146‐interacting proteins in the human proteome microarray. B) Fluorescent signal and signal‐noise ratio (SNR) of CD146‐interacting cytokines. C) Co‐immunoprecipitation (co‐IP) assay showing the interaction between CD146 and ANGPTL2 in mouse WAT. D) Co‐IP assay showing the enhanced interaction between CD146 and ANGPTL2 in WAT from obese mice. E,F) Pull‐down assay showing the direct interaction between CD146 and ANGPTL2. Purified His‐tagged ANGPTL2 and CD146 proteins were incubated in PBS. CD146 and ANGPTL2 were pulled down using anti‐CD146 antibodies E) and anti‐His tag antibodies F), respectively. G) Schematic diagram of the full‐length (FL) and CD146 truncates fused with Flag‐tag. H) Co‐IP assay showing that the fourth domain of CD146 is required for the ANGPTL2‐CD146 interaction. ANGPTL2 was coexpressed with different CD146 truncates in 293 cells. I) Pull‐down assay showing that anti‐CD146 AA98 antibodies blocked the CD146‐ANGPTL2 interaction.