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. 2021 Mar 9;13(5):1174. doi: 10.3390/cancers13051174

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The MEK/Raf/ERK signaling pathways are crucial for CAIX-induced cell migration and EMT. (A) The levels of total and phosphorylated MEK, Raf, and ERK1/2 in SiHa and Caski cells with or without CAIX expression were determined using Western blot analyses. b-Actin was used as a loading control. (B) After knockdown PFKFB4 expression, total and phosphorylated ERK1/2 expression was determined using Western blot analyses. (C,D) Boyden chamber assay and Western blot was used to analyze the migration and protein level of PD98059 treating in SiHa cells with CAIX overexpression. Scale bar, 100 μm. (*, p < 0.05, compared to pcDNA or Scramble siRNA; #, p < 0.05, compared to CAIX).