Figure 1.

Expression of the CREB3 and CREB3L2 proteins in HEK293 cells. (A–C) HEK293 cells were treated with thapsigargin (Tg, 0.01 μM), tunicamycin (Tm, 2 μg/mL), brefeldin A (BFA, 0.5 μg/mL), monensin (Mone, 1 μM), nigericin (Nigr, 0.2 μM), concanamycin A (CMA, 50 nM), cycloheximide (CHX, 20 μg/mL), MG132 (MG, 10 μM), or vehicle (Control (Con)) for 8 h. The expression of the indicated protein was detected as described in the Materials and Methods. Open and filled arrowheads indicate full-length mature and cleaved CREB3 and CREB3L2 proteins, respectively. Representative results of three independent cultures are shown.