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. Author manuscript; available in PMC: 2022 Jan 6.
Published in final edited form as: ACS Chem Neurosci. 2020 Dec 16;12(1):79–98. doi: 10.1021/acschemneuro.0c00561

Figure 2.

Figure 2.

Subunit-selective actions of EU1180–55 enantiomers. (A1,2) Left panel, representative whole cell current response from a HEK cell expressing GluN1/GluN2A to 100 μM glutamate and 30 μM glycine (plus vehicle, 0.075% DMSO), followed by an immediate switch to glutamate/glycine plus 15 μM (S)-EU1180–55 (A1) or (R)-EU1180–55 (A2). Middle panel, normalized mean whole cell current time course recorded in response to 1.5 s application of 100 μM glutamate and 30 μM glycine in the absence (black) and presence of 15 μM (S)-EU1180–55 (red) or (R)-EU1180–55 (blue); EU1180–55 and glycine were in the wash solution prior to application of glutamate. Right panel, Plot of weighted mean tau in vehicle or test compound. The same experiment is shown for GluN1/GluN2B (B1,2), GluN1/GluN2C (C1,2), and GluN1/GluN2D (D1,2). (E, F) Fold change in steady-state amplitude (E) or weighted tau (F) for (S)-EU1180–55 or (R)-EU1180–55. Fitted time constants for all experiments are given in Table 2 (weighted tau) and Supplemental Table S2 (tauFAST, tauSLOW, relative contribution for tauFAST). * p < 0.05, paired t test.