Figure 1.

RBE4 cell viability assay during ethanol (EtOH) treatment. RBE4 cells were incubated with increasing EtOH concentrations (10–1731 mM) for 30 min, 1, 2 and 4 h. Cell viability, quantified by MTT (3-(4,5-di-methylthiozol-2-yl)-2,5-diphenyltetrazolium bromide) assay (570 nm wavelength absorbance), decreased significantly (* p < 0.05) only at the highest EtOH concentrations. Values are expressed in percentage of control (untreated cells) absorbance as the mean ± S.E.M. of three independent experiments in quintuplicate. Grayscale refers to the increasing EtOH concentration. * p < 0.05 vs. control (untreated cells).