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. 2021 Mar 3;21(5):459. doi: 10.3892/etm.2021.9890

Figure 2.

Figure 2

miR-23 suppresses inflammation and binds to the 3'-UTR of CXCL12. (A) Transfection efficacy of miR-23 mimic and miR-23 inhibitor. ELISAs were performed to assess the effects of miR-23 overexpression and knockdown on the secretory levels of (B) TNF-α, (C) IL-1β and (D) IL-8. (E) The binding sequences between miR-23 and CXCL12 were predicted using StarBase. (F) The luciferase reporter assay was performed to verify the interaction between miR-23 and CXCL12. (G) CXCL12 expression levels were negatively regulated by miR-23 in FLS cells. *P<0.05. miR, microRNA; UTR, untranslated region; CXCL12, chemokine C-X-C motif ligand 12; WT, wild-type; Mut, mutant; NC, negative control.