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. 2021 Mar 3;21(5):459. doi: 10.3892/etm.2021.9890

Figure 4.

Figure 4

NF-κB signaling is crucial for miR-23-mediated regulation of inflammatory cytokine expression. (A) The effects of CXCL12 knockdown on the protein expression levels of p65, p-p65 and IκBα were measured via western blotting. (B) IκBα expression levels following transfection with siIκBα and siNC were measured via RT-qPCR. IκBα (C) mRNA and (D) protein expression levels were measured via RT-qPCR and western blotting, respectively. ELISAs were performed to measure the secretory levels of (E) TNF-α, (F) IL-1β and (G) IL-8. *P<0.05. miR, microRNA; CXCL12, chemokine C-X-C motif ligand 12; p, phosphorylated; IκBα, inhibitor κBα; si, small interfering RNA; NC, negative control; RT-qPCR, reverse transcription-quantitative PCR.