Figure 3.

SNHG1 directly inhibits miR-145 expression in hHCtASMCs. (A) The predicted target sequence in the miR-145 for SNHG1 binding. (B) In a hHCtASMC cell line, the luciferase activity of the SNHG1-WT reporter plasmid was decreased by miR-145 overexpression, while that of the SNHG1-MUT plasmid was not affected. (C) Knockdown of SNHG1 increased the expression levels of miR-145 in hHCtASMCs. ^*P<0.05 and ^***P<0.001 vs. control. miR, microRNA; si-SNHG1, short interfering RNA targeting small nucleolar RNA host gene 1; WT, wild-type; MUT, mutant; NC, negative control; hHCtASMCs, human carotid artery smooth muscle cells.