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. 2021 Mar 1;21(5):455. doi: 10.3892/etm.2021.9886

Figure 2.

Figure 2

KCNQ1OT1 may serve as a competitive endogenous RNA of miR-211-5p. (A) Binding sequence between KCNQ1OT1 and miR-211-5p was predicted by lncBase Predicted v.2. (B) Relative expression level of miR-211-5p after transfection with sh-KCNQ1OT1/NC or pcDNA-KCNQ1OT1/NC into C28/I2 cells was detected by RT-qPCR. **P<0.01 vs. sh-NC. (C) Targeting association between KCNQ1OT1 and miR-211-5p was validated by dual-luciferase reporter assay. (D) Targeting association between KCNQ1OT1 and miR-211-5p was validated by RIP assay. **P<0.01 vs. miR-NC. miR, microRNA; sh, short hairpin RNA; NC, negative control; KCNQ1OT1, KCNQ1 opposite strand/antisense transcript 1; wt, wild type; mut, mutant; RIP, RNA immunoprecipitation.