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. 2021 Mar 1;21(5):455. doi: 10.3892/etm.2021.9886

Figure 3.

Figure 3

Overexpression of miR-211-5p reduces chondrocyte injury caused by LPS in C28/I2 cells. (A) Relative expression level of miR-211-5p in OA cartilage tissues and normal cartilage tissues was determined by RT-qPCR. **P<0.01 vs. normal. (B) Relative expression level of miR-211-5p in C28/I2 cells was determined by RT-qPCR. **P<0.01 vs. control. (C) Relative expression level of miR-211-5p in C28/I2 cells was determined by RT-qPCR following transfection with miR-211-5p mimics. **P<0.01 vs. blank. (D) Cell viability was assessed by MTT assay. **P<0.01 vs. LPS + miR-NC. Levels of (E) IL-6, (F) IL-1β and (G) TNF-α in culture medium were measured by ELISA. **P<0.01 vs. LPS + miR-NC. (H) Protein expression levels of MMP-3 MMP-13, collagen X and collagen II were detected by western blotting. **P<0.01 vs. LPS + miR-NC. miR, microRNA; LPS, lipopolysaccharide; OA, osteoarthritis; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NC, negative control; MMP, matrix metalloproteinase.