Figure 1.

Emodin induces collagen synthesis in Hs27 dermal fibroblasts. (A) Immunoblotting of collagen type I following treatment with the YIGSR peptide and emodin. After 12-h treatment with 1 µM of each compound, cell lysates were electrophoresed and blotted. (B) Collagen type I and MMP-1 levels after dose-dependent emodin treatment for 12 h. (C) Transcript levels of various collagen types after emodin treatment. Normalization was performed using GAPDH. Values are represented as mean ± SEM. ^*P<0.05; ^**P<0.01; ^***P<0.001. Ct values are shown in the graph. NT, non-treated; MMP, matrix metalloprotease.