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Cell-survival rates are not affected by emodin treatment. (A) Survival of Hs27 cells after dose-dependent emodin treatment for 24 h. (B) Light microscopy images obtained after 1 µM emodin treatment for 12 h. Scale bar, 100 µm. (C) Following emodin treatment for 24 h, apoptosis was detected by FACS analysis using Annexin V/7-ADD double staining. N.S., not significant; NT, non-treated.
