| FRAP |
Fluorescent molecules in a small region are photobleached, and subsequent movement of surrounding nonbleached fluorescent molecules into the photobleached area is monitored (Lippincott-Schwartz et al., 2018). |
Svoboda et al., 1996; Bloodgood and Sabatini, 2005
|
| FCS |
Fluctuation of fluorescence intensity from the detection volume fixed at a specific position is recorded, and a temporal correlation is analyzed (Elson, 2011). |
Chen et al., 2015; Obashi et al., 2019
|
| RICS |
Spatial correlation is analyzed from raster-scanned images (Digman and Gratton, 2011). |
Obashi et al., 2019 |
| SPT |
The movement of a single particle is tracked using time-lapse imaging, and a trajectory is made and analyzed. To detect single particles, the density of fluorescence particles should be kept low (Shen et al., 2017). |
Borgdorff and Choquet, 2002; Varela et al., 2016
|
| SPT-PALM |
Only a small number of photoactivatable fluorescent proteins in the field of view are activated and tracked until they are bleached (Manley et al., 2010). |
Frost et al., 2010b; Nair et al., 2013
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