Figure 2.

Drug-resistant phenotype of AMC-HN-8 cells is modulated by EVs in vitro. (A) ABCG2 mRNA expression levels in EVs were detected by RT-qPCR. ABCG2 mRNA expression levels in EVs1 were significantly higher compared with those in EVs2. *P<0.01 vs. EVs2. (B) IC₅₀ value of CDDP in AMC-HN-8 cells following treatment with EVs. The IC₅₀ value of CDDP on AMC-HN-8 cells was significantly higher following treatment with EVs1 compared with that in cells treated with EVs2. *P<0.01 vs. AMC-HN-8-EVs1. (C-E) The mRNA and protein expression levels of ABCG2 in AMC-HN-8 cells following treatment with EVs. (C) ABCG2 mRNA expression levels in cells were detected by RT-qPCR. ABCG2 mRNA expression levels in the AMC-HN-8-EVs1 group were significantly higher compared with those in AMC-HN-8-EVs2 and AMC-HN-8 control groups. *P<0.01 vs. AMC-HN-8-EVs1. (D) ABCG2 protein expression levels in the AMC-HN-8-EVs1 group were significantly higher compared with those in the AMC-HN-8-EVs2 and AMC-HN-8 control groups. *P<0.01 vs. AMC-HN-8-EVs1. (E) Representative flow cytometry plots of ABCG2 protein expression in AMC-HN-8 cells. ABCG2, ATP-binding transporter G2; CDDP, cisplatin; EVs, extracellular vehicles; EVs1, EVs derived from AMC-HN-8/CDDP cells; EVs2, EVs derived from AMC-HN-8 cells; RT-qPCR, reverse transcription-quantitative PCR.