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. 2021 Mar 10;21(5):364. doi: 10.3892/ol.2021.12625

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Figure 3. — Cell cycle and apoptotic rate analysis of AMC-HN-8 cells following treatment with EVs. (A) The number of cell in the S phase and the cell PI were significantly higher in the AMC-HN-8-EVs1 group compared with those in the AMC-HN-8-EVs2 and control groups, whereas the numbers of cells in the G_0/1 and G₂/M phases were lower. (B) Representative flow cytometry plots of the cell cycle distribution of AMC-HN-8 cells following treatment with EVs. (C) Cells in the AMC-HN-8-EVs1 group exhibited a lower apoptotic rate compared with that in the AMC-HN-8-EVs2 and AMC-HN-8 groups. *P<0.01 vs. AMC-HN-8-EVs1. (D) Representative flow cytometry plots of AMC-HN-8 cells following 48-h treatment with EVs. ABCG2, ATP-binding transporter G2; CDDP, cisplatin; EVs, extracellular vehicles; EVs1, EVs derived from AMC-HN-8/CDDP cells; EVs2, EVs derived from AMC-HN-8 cells; PI, proliferation index.