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. 2021 Mar 16;12:186. doi: 10.1186/s13287-021-02256-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Upregulated EIF5A2 expression in OCSC-like cells. a Protein expression of EIF5A2, OCT-4, and ALDH1A1 of parental SKOV3, HO-8910 cells (monolayer), day 7 spheroid cells derived from SKOV3, HO-8910 cells growing in serum-free medium with growth factors (spheres), measured by western-blot. b Protein expression of EIF5A2, OCT-4, and ALDH1A1 of MCAs from two EOC ascites (spheres), adherent cells after re-differentiation growing in serum-complete medium (monolayer). c Immunofluorescent staining of CD133 (red), EIF5A2 (green), and their co-localization (yellow) in EOC malignant cell from ascites. d Correlation analysis demonstrating that overexpression of EIF5A2 was positively correlated with percent of ALDH+CD44+ subpopulation cells in MCAs from EOC patients. (r = 0.6268, p < 0.05). e Isolation of ALDH+CD44+ and ALDH−CD44− subpopulaiton cells by FACS. (Left) Isotype control. (Middle) The top 5% cells showing the highest staining for ALDH+CD44+ or bottom 5% with minimal staining for ALDH-CD44- cells were collected. (Right) Purity of ALDH and CD44 was confirmed via post sorting using flow cytometry. f Relative EIF5A2 mRNA and protein expression of ALDH+CD44+ and ALDH-CD44- subpopulation cells in MCAs #5, MCAs #9, and SKOV3 cells via qPCR and immunoblotting, respectively. All the data represent the means ± SD; *p < 0.05, **p < 0.01, and ***p < 0.001