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. 2021 Mar 15;131(6):e129433. doi: 10.1172/JCI129433

Figure 12. E2 or ER-α agonist PPT rescues MCT-induced PH and increases RV BMPR2 and apelin.

Figure 12

(A) Experimental design. (B) Western blot analysis of RVs from MCT-PH rats. A representative Western blot is depicted on top of panel; densitometric analysis from all experimental animals is shown at bottom. (C–F) Effects of E2 or PPT on RV systolic pressure (RVSP; C), RV hypertrophy (RV weight divided by weight of left ventricle plus septum; RV/[LV + S]; D), cardiac index (echocardiographic cardiac output/body weight; E), and total pulmonary resistance index (cardiac index/RVSP; F). (G) Time course of cardiac index. Percentage change in cardiac index versus 2-week baseline time point is shown in legend in parentheses behind group names. *P < 0.05 versus control, #P < 0.05 versus untreated MCT (1-way ANOVA with Tukey or Dunnett’s post hoc correction). Each data point in B–F represents 1 male animal. Error bars represent mean ± SEM. Sq = subcutaneous.