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An integrated approach for top-down MS characterization of endogenous membrane proteins. A cloud point extraction procedure is performed using Triton X-114 or Tergitol NP-7 to extract and enrich endogenous membrane proteins from cells or tissues. Next, an SEC separation is performed for removal of detergent and protein fractionation. Finally, reversed-phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) is used to obtain sequence information for protein identification.
