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. 2021 Mar 16;21:171. doi: 10.1186/s12935-021-01865-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — ANGPTL4 expression is highest in A2780 and CAOV3 ovarian cancer cells and tumors. a Western blot and statistical graph of angiopoietin-like 4 (ANGPTL4) expression in human ovarian clear cell carcinoma ES2 cells, human ovarian carcinoma A2780 and NCI/ADR-RES cells, and human ovarian adenocarcinoma COC1, SKOV3, OVCAR3, and CAOV3 cells; n = 3 independent experiments per group. b qRT-PCR analysis was performed to determine the expression pattern of ANGPTL4 mRNA in human ovarian cancer cells; n = 3 independent experiments per group. c Immunofluorescence analysis and statistical graph of ANGPTL4 expression in ovarian cancer cells; n = 3 per group. Scale bar: 50 μm. d Western blot and statistical graph of ANGPTL4 expression in the A2780 and CAOV3 xenograft models; n = 4 independent experiments per group. e qRT-PCR analysis of ANGPTL4 mRNA expression was also carried out in A2780 and CAOV3 tumors; n = 4 independent experiments per group. ***p < 0.001 for the indicated comparisons. The data are presented as the mean ± SEM values