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. 2021 Mar 16;21:171. doi: 10.1186/s12935-021-01865-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Analysis of the VEGFR2/VE-cadherin/Src complex in A2780 and CAOV3 tumors treated with or without siRNA targeting ANGPTL4. a Immunoprecipitation (IP) of vascular endothelial growth factor receptor 2 (VEGFR2) and immunoblotting (IB) of VE-cadherin, Src kinase phosphorylated at tyrosine 416 (Src pY416), Src, and VEGFR2. Total lysates were immunoblotted for VEGFR2 phosphorylated at tyrosine 949 (VEGFR2 pY949), VEGFR2, VE-cadherin, Src pY416, Src, and GAPDH; n = 3 independent experiments per group. b VE-cadherin/VEGFR2 ratio in immunoprecipitates from A2780 and CAOV3 tumors. c The Src pY416 level normalized to that of GAPDH or Src and the Src/GAPDH ratio were determined in immunoblots of A2780 and CAOV3 total lysates. d The VEGFR2 pY949 level normalized to that of GAPDH or VEGFR2 and the VEGFR2/GAPDH ratio were analyzed in total lysates by immunoblotting. e VE-cadherin/GAPDH ratio in immunoblots of A2780 and CAOV3 total lysates. **p < 0.01, ***p < 0.001 for the indicated comparisons. ns indicates no significance. The data are presented as the mean ± SEM values