Figure 8.

Reduced 4E-BP1 expression reduces mitochondrial superoxide production and mitochondrial biogenesis in ADPKD in vitro models. PKD1^+/+, PKD1^−/− and Human Cyst 5 were transduced with 4E-BP1 targeting shRNA as described. (A) Mitochondrial O₂⁻ production (MitoSOX, Red, Nuclear co-stain DAPI, Blue) and (B) mitochondria quantification (ACAA2, Red; 4E-BP1, Green; Nuclear co-stain DAPI, Blue) were determined in neon, 4E-BP1^F113A and 4E-BP1^shRNA-transduced cell lines. Relative fluorescent unit (RFU). Scale bar (white): 20 μm. MitoSOX data was generated in a minimum of three experiments. Multiple-group comparisons are performed ANOVA with posttest according to Newman–Keuls. Values are expressed as the mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, #P < 0.0001. Not significant, NS.