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. 2020 Oct 19;162(4):341–356. doi: 10.1111/imm.13266

Table 3.

Studies analysing microbiota population dynamics in vivo

Bacterium Method Number of unique identifiers Analysed parameters Main finding References
E. coli, Klebsiella michiganensis (K. michiganensis) Fluorescent proteins 2 Intestinal colonization K. michiganensis provides colonization resistance against E. coli 52
E. coli Fluorescent proteins 2 Niche competition in intestinal colonization Horizontal gene transfer promotes niche adaptation 60
Microbiota 16S sequencing, qPCR quantification n.a. Replication and death rate of microbiota exposed to bile salts Diet‐evoked increased bile salt concentrations perturb microbiota composition and lead to decreased colonization resistance to S. Typhimurium 59
Several microbiota members (mouse) qPCR (16S) n.a. Replication rate, interstrain interactions Colonization dynamics in the murine intestine 58
Several microbiota members (fruitfly) Plating n.a. Transition time through intestine, intra‐intestinal replication and death rate Colonization dynamics in the intestine of Drosophila melanogaster 57

bp = base pairs, n.a. = not applicable.