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. Author manuscript; available in PMC: 2021 Mar 17.
Published in final edited form as: Clin Cancer Res. 2020 Jun 25;26(18):4970–4982. doi: 10.1158/1078-0432.CCR-19-3890

Figure 2: Levels of HAF and the HIFs during ccRCC progression.

Figure 2:

A) Representative images of large sections of paired uninvolved and T stage 1 tumor sections stained for HAF, HIF-2α and HIF-1α. Scale bars = 100μM. B-D) Quantitation of staining percent nuclear positivity of large tissue sections from T stage 1 tumors containing both tumor and uninvolved tissue (B), multi-grade tumor microarrays (C), and large tissue sections (D), based on an arbitrary threshold for HAF, HIF-2α and HIF-1α as determined by Aperio digital imaging software. Each dot on the plots indicates a single patient with patient numbers indicated in brackets. Average values ± SEM are shown with p-values indicated for (B), students’ paired T-tests, or (C,D) Mann Whitney U- tests. ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05. NS: not significant.