Table 2.

Analytical performance of BioFire® FilmArray® Pneumonia plus Panel compared to culture, taking into account microbiological thresholds (A) and irrespective of thresholds (B). Se, sensitivity; Sp, specificity; PPV, positive predictive value; NPV, negative predictive value

	Organisms	True positive (culture = mPCR)	False positive (mPCR +/culture −)	False negative (culture +/mPCR −)	True negative (culture +/mPCR −)	Se (%) [95% CI]	Sp (%) [95% CI]	PPV (%) [95% CI]	NPV (%) [95% CI]
A
Gram −	Escherichia coli	4	3	0	105	100.0	97.2	57.1	100.0
	Enterobacter cloacae complex	2	0	0	110	100.0	100.0	100.0	100.0
	Klebsiella aerogenes	4	2	0	106	100.0	98.1	66.7	100.0
	Klebsiella oxytoca	0	0	0	112	-	100.0	-	100.0
	Klebsiella pneumoniae group	2	4	0	106	100.0	96.4	33.3	100.0
	Proteus spp.	0	0	0	112	-	100.0	-	100.0
	Serratia marcescens	0	2	0	110	-	98.2	0.0	100.0
	Acinetobacter calcoaceticus-baumannii complex	1	2	0	109	100.0	98.2	33.3	100.0
	Pseudomonas aeruginosa	6	5	0	101	100.0	95.3	54.5	100.0
	Haemophilus influenzae	1	1	0	110	100.0	99.1	50.0	100.0
	Moraxella catarrhalis	0	0	0	112	-	100.0	-	100.0
Total		20	19	0	1193	100.0	98.4	51.3	100.0
Gram +	Staphylococcus aureus	5	4	0	103	100.0	96.3	55.6	100.0
	Streptococcus pneumoniae	0	0	0	112	-	100.0	-	100.0
	Streptococcus agalactiae	0	0	0	112	-	100.0	-	100.0
	Streptococcus pyogenes	0	0	0	112	-	100.0	-	100.0
Total		5	4	0	439	100.0	99.1	55.6	100.0
Atypical	Chlamydia pneumoniae	0	0	0	112	-	100.0	-	100.0
	Legionella pneumophila	0	0	0	112	-	100.0	-	100.0
	Mycoplasma pneumoniae	0	0	0	112	-	100.0	-	100.0
Total		0	0	0	336	-	100.0	-	100.0
Total		25	23	0	1968	100.0 [100.0-100.0]	98.8 [98.4-99.3]	52.1 [38.0-66.2]	100.0 [100.0-100.0]
B
Gram −	Escherichia coli	7	0	2	103	77.8	100.0	100.0	98.1
	Enterobacter cloacae complex	2	0	0	110	100.0	100.0	100.0	100.0
	Klebsiella aerogenes	5	1	1	105	83.3	99.1	83.3	99.1
	Klebsiella oxytoca	0	0	0	112	-	100.0	-	100.0
	Klebsiella pneumoniae group	4	2	1	105	80.0	98.1	66.7	99.1
	Proteus spp.	0	0	2	110	0.0	100.0	-	98.2
	Serratia marcescens	2	0	0	110	100.0	100.0	100.0	100.0
	Acinetobacter calcoaceticus-baumannii complex	3	0	1	108	75.0	100.0	100.0	99.1
	Pseudomonas aeruginosa	11	0	1	100	91.7	100.0	100.0	99.0
	Haemophilus influenzae	1	1	0	110	100.0	99.1	50.0	100.0
	Moraxella catarrhalis	0	0	0	112	-	100.0	-	100.0
Total		35	4	8	1185	81.4	99.7	89.7	99.3
Gram +	Staphylococcus aureus	8	1	1	102	88.9	99.0	88.9	99.0
	Streptococcus pneumoniae	0	0	0	112	-	100.0	-	100.0
	Streptococcus agalactiae	0	0	0	112	-	100.0	-	100.0
	Streptococcus pyogenes	0	0	0	112	-	100.0	-	100.0
Total		8	1	1	438	88.9	99.8	88.9	99.8
Atypical	Chlamydia pneumoniae	0	0	0	112	-	100.0	-	100.0
	Legionella pneumophila	0	0	0	112	-	100.0	-	100.0
	Mycoplasma pneumoniae	0	0	0	112	-	100.0	-	100.0
Total		0	0	0	336	-	100.0	-	100.0
Total		43	5	9	1959	82.7 [71.4-94.0]	99.7 [99.5-100.0]	89.6 [80.9-98.2]	99.5 [99.2-99.8]