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. 2021 Jan 21;41(3):240–257. doi: 10.1002/cac2.12127

FIGURE 3.

FIGURE 3

BAALC‐AS1 overexpression promotes cell proliferation in KYSE‐180 and KYSE‐410 cells. A. The expression levels of BAALC‐AS1 in BAALC‐AS1‐overexpressing KYSE‐180 and KYS‐410 cells were assessed by RT‐qPCR. B. MTS assay was used to determine the cell viability in BAALC‐AS1‐overexpressing KYSE‐180 and KYS‐410 cells. C. BrdU incorporation shows the synthesis of DNA in BAALC‐AS1‐overexpressing KYSE‐180 and KYS‐410 cells. D. Flow cytometry detected the number of BAALC‐AS1‐overexpressing KYSE‐180 and KYS‐410 cells in each phase of the cell cycle (left panel). The percentages of BAALC‐AS1‐overexpressing KYSE‐180 and KYS‐410 cells are increased in S and G2/M phases (right panel). E. Western blotting analyses of the protein expression levels of Cyclin D, Cyclin E, CDK2 and CDK4 in BAALC‐AS1‐overexpressing KYSE‐180 and KYS‐410 cells. Vector, cells were infected with empty lentiviruses; BAALC‐AS1, cells were infected with lentiviruses expressing BAALC‐AS1. *, P < 0.05; **, P < 0.01. All values are expressed as mean ± SEM of 3 experiments.