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. 2021 Feb 8;10:e62873. doi: 10.7554/eLife.62873

Figure 5. TWIST1 is required for the recruitment of CHD8 to the regulatory region of target genes.

Figure 5.

Binding of endogenous TWIST1 and CHD8 to overlapping genomic peak regions called by MACS2 (q < 0.05) were assessed by ChIP-qPCR. (A–E) qPCR quantification of genomic DNA from ChIP of endogenous TWIST1 or CHD8 proteins are shown as mean fold enrichment ± SE. ChIP experiments using anti-TWIST1 or anti-CHD8 antibodies against endogenous proteins were performed on wildtype (WT), Twist1+/- and Chd8+/- NECs derived from ESC (n = 3, day 3). qPCR results were normalized against signal from non-binding negative control region and displayed as fold change against IgG control. Each condition was compared against WT and P-values were generated using one-way ANOVA. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. ns, not significant.