Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Aantibody (mouse monoclonal) |
α-TWIST | Abcam | cat# ab50887; RRID:AB_883294 |
1:1000 |
| Antibody (rabbit polyclonal) |
α-CHD7 | Abcam | cat# ab117522; RRID:AB_10938324 |
1:5000 |
| Antibody (rabbit polyclonal) |
α-CHD8 | Abcam | cat# ab114126; RRID:AB_10859797 |
1:10,000 |
| Antibody (mouse monoclonal) |
α-WHSC1 | Abcam | cat# ab75359; RRID:AB_1310816 |
1:5000 |
| Antibody (rabbit polyclonal) |
α-SOX2 | Abcam | cat# ab59776; RRID:AB_945584 |
1:1000 |
| Antibody (mouse monoclonal) |
α-VINCULIN | Sigma | cat# V9131; RRID:AB_477629 |
1:1000 |
| Antibody (mouse monoclonal) |
α-OCT3/4 | Santa Cruz | cat# sc-5279; RRID:AB_628051 |
1:1000 |
| Antibody (rabbit polyclonal) |
α-TFAP2A | Abcam | cat# ab52222; RRID:AB_867683 |
1:1000 |
| Antibody (mouse monoclonal) |
α-NESTIN | Abcam | cat# ab7659; RRID:AB_2298388 |
1:1000 |
| Antibody (mouse monoclonal) |
α-FLAG | Sigma | cat# F1804; RRID:AB_262044 |
1:1000 |
| Antibody (mouse monoclonal) |
α-neurofilament | DSHB | cat#2H3; | 1:1000 |
| Antibody (rabbit polyclonal) |
α-HA | Abcam | cat# ab9110; RRID:AB_307019 |
1:1000 |
| Transfected construct (M. musculus) |
siChd7 #1 | Sigma-Aldrich | SASI_Mm02_00298181 | |
| Transfected construct (M. musculus) |
siChd7 #2 | Sigma-Aldrich | SASI_Mm01_00234434 | |
| Transfected construct (M. musculus) |
siChd7 #3 | Sigma-Aldrich | SASI_Mm01_00234436 | |
| Transfected construct (M. musculus) |
siChd8 #1 | Sigma-Aldrich | SASI_Mm02_00351688 | |
| Transfected construct (M. musculus) |
siChd8 #2 | Sigma-Aldrich | SASI_Mm02_00351689 | |
| Transfected construct (M. musculus) |
siChd8 #3 | Sigma-Aldrich | SASI_Mm02_00351691 | |
| Transfected construct (M. musculus) |
siWhsc1 #1 | Sigma-Aldrich | SASI_Mm01_00278608 | |
| Transfected construct (M. musculus) |
siWhsc1 #2 | Sigma-Aldrich | SASI_Mm01_00278610 | |
| Transfected construct (M. musculus) |
siWhsc1 #3 | Sigma-Aldrich | SASI_Mm02_00295201 | |
| Transfected construct (M. musculus) |
siTwist1 | Sigma-Aldrich | SASI_Mm01_00043025 | |
| Transfected construct (M. musculus) |
siRNA Universal Negative Control #1 |
Sigma-Aldrich | SIC001 | |
| Transfected construct (M. musculus) |
siRNA Universal Negative Control #2 |
Sigma-Aldrich | SIC002 | |
| Cell line (M. musculus) |
O9-1 neural crest stem cell |
Millipore | Millipore | |
| Cell line (M. musculus) |
3T3 | ATCC | ATCC | |
| Cell line (M. musculus) |
A2loxCre ESCs | Kyba Lab | Lillehei Heart Institute, Minnesota, USA |
|
| Cell line (M. musculus) |
A2loxCre Twist1+/- | This paper | See Materials and methods | |
| Cell line (M. musculus) |
A2loxCre Twist1-/- | This paper | See Materials and methods | |
| Cell line (M. musculus) |
A2loxCre Twist1+/-;
Chd7+/- |
This paper | See Materials and methods | |
| Cell line (M. musculus) |
A2loxCre Twist1+/-;
Chd8+/- |
This paper | See Materials and methods | |
| Cell line (M. musculus) |
A2loxCre Twist1+/-;
Whsc1+/- |
This paper | See Materials and methods | |
| Cell line (M. musculus) |
A2loxCre Chd7+/- | This paper | See Materials and methods | |
| Cell line (M. musculus) |
A2loxCre Chd8+/- | This paper | See Materials and methods | |
| Cell line (M. musculus) |
A2loxCre Whsc1+/- | This paper | See Materials and methods |