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. 2021 Mar 17;10:e63329. doi: 10.7554/eLife.63329

Video 3. Ca2+ dynamics following Gq-DREADD-activation.

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Example video of 20 min of in vivo GCaMP activity in layer 2/3 V1 cortical astrocytes expressing Gq-DREADDs. Video begins immediately after 1 mg/kg CNO administration, demonstrating the initial period of elevated Ca2+, followed by almost complete Ca2+ suppression. Frame rate = 30 Hz. Scale bar = 50 μm.