Table 1.

Kinetic constants of D135T and D135S demethylating m¹G in the RNA oligo 5′-GAGC(m¹G)UUAG

Enzyme	k cat (min−1)	K M (μM)	k cat/KM (M−1min−1)
D135T	0.052 ± 0.008	3.3 ± 1.3	15.7 ± 3.7
D135S	-	-	2.2