Table 1.
Data collection and structure refinement statistics
| RBM45RRM1–2 | RBM45RRM1–2−ssDNA | |
|---|---|---|
| Data collection | ||
| Wavelength (Å) | 0.9792 | 0.9793 |
| Space group | P43 | P43 |
| Cell dimensions | ||
| a, b, c (Å) | 62.48, 62.48, 52.02 | 86.55, 86.55, 27.34 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 |
| Resolution (Å) | 50.00−2.50 (2.59−2.50)* | 50.00−1.80 (1.86−1.80) |
| R merge | 0.160 (0.950) | 0.091(1.391) |
| I/σI | 16.4 (3.0) | 21.3 (1.7) |
| CC1/2 | 0.982 (0.810) | 0.999 (0.764) |
| Completeness (%) | 99.7 (99.3) | 100.0 (100.0) |
| Redundancy | 10.9 (9.2) | 7.3 (7.4) |
| Total/Unique reflections | 76 581/7056 | 141 140/19410 |
| Refinement | ||
| Resolution (Å) | 44.18−2.50 | 21.64−1.80 |
| No. reflections | 7029 | 19273 |
| R work/Rfree | 0.201/0.255 | 0.185/0.234 |
| No. atoms | ||
| Protein | 1366 | 1413 |
| DNA | / | 212 |
| Water | 86 | 169 |
| B-factors (Å2) | ||
| Protein | 38.0 | 43.9 |
| DNA | / | 63.4 |
| Water | 37.3 | 50.1 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.002 | 0.004 |
| Bond angles (°) | 0.514 | 0.596 |
| MolProbity score | 1.27 | 0.79 |
| Ramachandran plot | ||
| Favored | 98.2% | 98.3% |
| Allowed | 1.8% | 1.7% |
| Outliers | 0 | 0 |
*Values in parentheses are for the highest-resolution shell.