Table 2.
Usage of individual dBPs in chicken intron 4a
| DBR1-deficient cells | Number of Sanger-sequenced clones | Number of informative clones (%) | dBP+23 (dBP+25) | dBP+29 (dBP+31) | dBP+34 (dBP+36) | dBP+39 (dBP+41) |
|---|---|---|---|---|---|---|
| Lacking TIA-1 and TIAR | 13 | 12 | 0 | 0 | 5 | 7 |
| Overexpressing PUF60 | 14 | 14 | 0 | 0 | 2 | 12 |
| Controls | 17 | 14 | 1 | 1 | 2 | 10 |
| Total | 44 | 40 | 1 | 1 | 9 | 29 |
χ2 for 2 × 4 contingency tables comparing the usage of human (Table 1) and chicken dBP orthologues was 37.6 (P< 10−9) for controls and 95.5 (P< 10−16) for all clones. Aligned sequences of informative clones are in Supplementary Figure S3E.