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. 2021 Feb 12;49(5):2721–2739. doi: 10.1093/nar/gkab078

Figure 1.

Figure 1.

PS-ASO binding can alter the stability of RNase H1 protein. (A) Thermostability assay was employed to determine the melting curve of RNase H1-NTD incubated without or with 5–10–5 gapmer PS-ASOs of the same sequence but modified with 2′-MOE (ASO 116847), 2′-cEt (ASO 582801) or 2′-Fluoro (ASO 404130). The top panel showed the real curves for the different groups; the bottom panel showed the derivatives of the melting curves. The vertical dashed lines indicate the Tms. (B) The protein melting temperature (Tm) of H1-NTD is determined using the protein thermostability assay (PTS). There are four repeats (red dots) for each experimental group; the green line represents the median; diamond shows the 95% lower and upper confidence limit. (C) Melting temperature (Tm, in °C) of RNase H1 domains complexed with ss-PS-ASOs or ASO/RNA duplexes, as determined by thermostability shift assay. The average values and standard deviations from four duplicates are shown.