Table 1.
Magnesium and aminoglycoside effects on GTP hydrolysis for tRNAGlu reading unmodified and m6A-modified codons. Related to Figure 2A, B and Supplementary Figure S1. Par, paromomycin; Neo, neomycin
| (kcat/Km)GTP (μM−1s−1) at varying Mg2+ concentration | ||||||
|---|---|---|---|---|---|---|
| Codon | Aminoglycoside | 2.3 mM | 5.3 mM | 9.8 mM | 16 mM | 25 mM |
| GAA | No | 59 ± 7 | 150 ± 19 | 193 ± 26 | 210 ± 22 | 200 ± 32 |
| GAU | No | 0.98 ± 0.11 | 5.9 ± 0.9 | 9.1 ± 1.3 | 20 ± 0.8 | 20 ± 1.5 |
| GAA | 10 μM Par | 68 ± 6.7 | 158 ± 18 | 207 ± 15 | 186 ± 10 | 212 ± 24 |
| GAU | 10 μM Par | 9.3 ± 0.92 | 29 ± 2.8 | 37 ± 4.6 | 48 ± 4.7 | 53 ± 6.3 |
| GAU | 10 μM Neo | – | – | – | – | 79 ± 7.6 |
| Gm6AA | No | 5.6 ± 0.55 | 13 ± 1.8 | 18 ± 1.7 | 20 ± 1.1 | – |
| Gm6AU | No | 0.38 ± 0.02 | 2.7 ± 0.24 | 8.2 ± 1.1 | 11 ± 0.7 | – |
| Gm6AA | 10 μM Par | 7.02 ± 0.9 | 15 ± 1.5 | 14 ± 1.1 | 20 ± 0.8 | – |
| Gm6AU | 10 μM Par | 3.2 ± 0.34 | 11 ± 1.2 | 13 ± 1.4 | 21 ± 1.4 | – |