Table 2.
Kinetic parameters of peptide release by RF2 on m6A-modified and unmodified codons. Accuracy (A) is the ratio of efficiencies (kcat/Km) on respective codons
| Rate constants of peptide release | Accuracy (A) | ||||
|---|---|---|---|---|---|
| Codon | k cat (s−1) | k cat/Km (μM−1 s−1) | K m (μM) | Codon pair | Accuracy |
| UAA | 2.8 ± 0.18 | 70 ± 13 | 0.04 ± 0.008 | UAA / UAG | 12000 ± 3000 |
| Um6AA | 3.0 ± 0.06 | 11 ± 0.5 | 0.27 ± 0.014 | Um6AA / Um6AG | 6500 ± 500 |
| UAG | 0.06 ± 0.006 | 0.006 ± 0.001 | 10 ± 2 | UAA / Um6AA | 6.4 ± 1.2 |
| Um6AG | 0.027 ± 0.007 | 0.0017 ± 0.0001 | 16 ± 1 | UAG / Um6AG | 3.5 ± 0.8 |