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. 2021 Feb 22;49(5):2740–2758. doi: 10.1093/nar/gkab081

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — CHIP is overexpressed in cancer cells and human prostate tumors. CHIP and Hsp70 are associated with low overall survival in human cancers. (A) CHIP is overexpressed in squamous cell carcinoma cell lines. Cell extracts were prepared and immunoblotted with TAp63-specific (Poly 618902), ΔNp63-specific (Poly 619002), CHIP-specific (H-231), Hsp70-specific (C92F3A5), Itch-specific (ab109018), MDM2-specific (SMP14) antibodies. The inverse correlation between TAp63 and CHIP protein levels was tested with a Pearson correlation using OriginPro 2019 software. Pearson correlation: –0.7188; significance (two-tailed test): P < 0.05. (B) Similar to (A), except that melanoma cell lines were used. The inverse correlation between TAp63 and CHIP protein levels was tested with a Pearson correlation. Pearson correlation: –0.85895; significance (two-tailed test): P < 0.05. (C) Whole lysates were harvested from nine invasive prostate carcinoma tissue samples and two benign prostate neoplasm samples as indicated and analyzed by western blotting with the antibodies against TAp63 (Poly 618902), ΔNp63 (Poly 619002), CHIP (H-231), and Hsp70 (C92F3A5). T denotes tumor tissues. The inverse correlation between TAp63 and CHIP protein levels was tested with a Pearson correlation. Pearson correlation: –0.9196; significance (two-tailed test): P < 0.01. An antibody to actin was used as a loading control. (D–F). Kaplan–Meier survival curves generated from www.genomicscape.com revealed that patients with high levels of CHIP and Hsp70 with lower levels of TP63 had a significantly lower overall survival in the Lenz, diffuse large B cell lymphoma. (G–J). Kaplan–Meier survival curves generated from www.genomicscape.com revealed that patients with high levels of CHIP and Hsp70 with low levels of TP63 had a significantly lower overall survival in human colon cancers. Please note the number: 209863 (D), 227625 (E), 227650 (F), 211834 (G), 217934 (H), 200799 (I), and 200800 (J) represented different ‘Affymetrix ID’ number.