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. 2021 Feb 22;49(5):2973–2984. doi: 10.1093/nar/gkab105

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — The assembly of the Cas4–Cas1 complex. (A) SEC/MALS analysis to measure the molecular weight of the Cas4–Cas1 complex. The molecular weight was determined to be 99.8 kDa, with 1% uncertainty. (B) EM envelop of the Cas4–Ca1 complex fitted with crystal structure of Cas1 and structural model of Cas4. Cas1 N-terminal domain, Cas1 C-terminal domain, and Cas4 are colored in red, yellow and blue, respectively. (C) MBP pull-down assays for the interactions between purified full-length Cas1 (Cas1-FL), C-terminal domain of Cas1 (Cas1-C), and MBP-tagged Cas4.