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. 2021 Feb 24;49(5):2403–2417. doi: 10.1093/nar/gkab080

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The ratio of TIA-1 protein:binding sites finely tunes liquid-liquid phase separation and fibril formation. (A) ssDNA with 1–9 TC binding sites used in this study. (B, C) Measuring the effect of DNA length at constant TC ssDNA:TIA-1 binding site ratio: (B) C_sat: Measurement of the concentration of dispersed TIA-1 from 10 μM alone or in the presence of DNA (0.5 μM TC5) with constant TIA-1:TC site ratio of 4:1. (C) Thioflavin-T assay of TIA-1 (2.5 μM) showing effect of ssDNA length when adjusted to constant TIA-1:TC site ratio of 2:1. (D) Turbidity assay of TIA-1 (2.5 μM) with increasing amount of TC5 (0.125–5 μM). (E) Thioflavin-T assay of TIA-1 (2.5 μM) alone (black) or in the presence of increasing concentrations of ssDNA TC5 (0.125–5 μM). ThT fluorescence was baseline corrected against ssDNA alone.