Skip to main content
. 2021 Feb 24;49(5):2403–2417. doi: 10.1093/nar/gkab080

Figure 5.

Figure 5.

Tandem TIA-1 binding sites in 3′ UTR of p53 mRNA induce phase separation of TIA-1. (A) TIA-1 binding sites present in p53 3′UTR. (B) DIC microscopy of TIA-1 alone or in presence of p53 ssRNA oligonucleotides. (C) Turbidity assay of TIA-1 alone or in the presence of p53 oligonucleotides maintaining 5:1 TIA-1:RNA ratio. (D) Thioflavin T assay of TIA-1 (2.5 μM) alone, or in the presence of p53 site 1 or 2 (0.25 μM) monitored at 30 °C. (E) Turbidity assay of TIA-1 (2.5 μM) alone or in the presence of p53 3′UTR RNA (0.25 nM) wild type (WT) or with TIA-1 RNA binding sites mutated (MT). (F) Thioflavin-T assay of TIA-1 and p53 3′ UTR from E.