Table 1.
Sample | Conc (mg/ml) | R G (Guinier) (Å) | I 0 (Guinier) | R 2 | R g (PDDF) (Å) | R max (Å) | Calculated MW (kDa) | MW from SAXSa (kDa) |
---|---|---|---|---|---|---|---|---|
Apo-TIA-1 | 2.9–0.37 | 31.92 ± 0.51 | 0.051 ± 3.3e-4 | 0.98 | 33.56± 0.26 | 127 | 42.2 | 37.7 |
TIA-1/TC1 | 2.9–0.37 | 32.41 ±0.57 | 0.068 ± 2.9e-4 | 0.97 | 35.17± 0.33 | 151 | 45.2 | 43.8 |
TIA-1/UC1 | 2.9–0.37 | 32.6 ±0.07 | 0.069 ± 0.0003 | 0.94 | 34.57± 0.34 | 144 | 45.2 | 41.9 |
aCalculated by Bayesian Inference according to: Hajizadeh, N.R., Franke, D., Jeffries, C.M. & Svergun, D.I. (2018) Consensus Bayesian assessment of protein molecular mass from solution X-ray scattering data. Sci Rep8, 7204.
Note: R2 = correlation of coefficient.