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. 2021 Feb 22;49(5):2959–2972. doi: 10.1093/nar/gkab076

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — The DNA-binding mode of HIN domains in PYHIN family. (A) FP assays of the DNA-binding interface of p204 HINab and HINa binding to 30 mer dsDNA. The apparent K_d values (K_d^a) are shown for p204 HINab, HINa and mutants. ND means not determined. (B) Structural comparison of HIN domains in complex with DNA. Structures of p204 HINa:DNA (orange) and HINb: DNA (slate) are from HINab:dsDNA complex. Structures of p202 HINa (PDB: 4L5R), human AIM2 HIN (PDB: 3RN2), murine AIM2 HIN (PDB: 4JBM), IFI16 HINa (PDB: 4QGU) and IFI16 HINb (PDB: 3RNU) are shown in salmon, pink, cyan, yellow orange and light blue, respectively. (C) Positive surface electrostatics of p204 HINa facing the DNA from the complexc of IFI16 HINa:DNA. It was set to 20% transparency. The loop between α3 and IIβ1, and L45 of OB2 on p204 HINa are labeled.