Extended Data Fig. 6. ¹³C¹⁵N-labelled EAAs are used for GSH synthesis in starving D. discoideum.

a, Total pools of GSH, GSSG, cysteine, glutamate and glycine in starved cells with or without EAA supplementation (n = 3). b, LC–MS metabolite tracing analysis of ¹³C and ¹⁵N incorporation from ¹³C¹⁵N-labelled EAAs into vegetative or starved D. discoideum (n = 3). The schematic shows incorporation of ¹³C and ¹⁵N into intermediate metabolites of GSH from amino acids. c, LC–MS metabolite tracing analysis of ¹³C-glucose into GSSG (n = 3). d, Percentage fractional contribution of ¹³C-glucose to GSSG labelling in vegetative and starved D. discoideum (n = 3). In a–d, data are mean ± s.e.m. n represents independent biological replicates. Statistical significance was calculated using a two-tailed Student’s t-test.

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