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. 2021 Feb 24;591(7850):471–476. doi: 10.1038/s41586-021-03270-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, UMAP clustering analysis of single-cell RNA-seq data from vegetative and starved D. discoideum cultured for the indicated times demonstrates populations of D. discoideum with distinct patterns of gene expression. b, Violin plots of established vegetative and developmental marker genes, predictive of the vegetative or developmental state of each cluster. c, Violin plots of mitochondrial genes expressed in each cluster, with unique molecular identifiers and feature counts. d, Bulk RNA-seq analysis showing increased expression of developmental genes during starvation, with decreased expression of genes for mitochondrial components (including OXPHOS complexes and structural membrane proteins). Single-cell and bulk RNA-seq (a–d) were performed using three independent biological replicates.