Extended Data Fig. 3. Starving Dictyostelium decrease anabolic and increase catabolic processes.
a, Bulk RNA-seq analysis of ribosomal and proteasomal genes in vegetative and starved D. discoideum (n = 3). b–d, Dictyostelium discoideum cultured with rapamycin (n = 6) (b), 3-methyladenine (3MA) (n = 6) (c) or bafilomycin (n = 3) (d) from starvation initiation. e, Western blot of OXPHOS complexes in vegetative or starved D. discoideum, with or without 3MA (n = 3). f, Relative proteasome activity in vegetative, starved or EAA-supplemented D. discoideum, with or without MG132 (n = 3). g, Western blot of OXPHOS complexes in vegetative or starved D. discoideum, with or without MG132 (n = 3). h, Seahorse analysis of starved D. discoideum, with or without MG132 (n = 3). In f, data are mean ± s.e.m. In h, data are mean ± s.d. n represents independent biological replicates. Statistical significance was calculated using a two-tailed Student’s t-test.