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. 2021 Mar 12;14(3):dmm046789. doi: 10.1242/dmm.046789

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — H3K27me3 and EZH2 enrichment at the Tbx1 locus, and gene expression. (A) qChIP experiments using an anti H3K27me3 antibody on three loci of the Tbx1 gene on chromatin from whole E9.5 mouse embryos, wild type (WT), Tbx1^+/− (T), and Tbx1^+/−;Ezh2^+/− (TE). Significant differences in H3K27me3 were detected at intron 1 (increased in T) and exon 3 loci (reduced in TE compared to T). (B) EZH2 qChIP using E9.5 mouse embryos. Note a significant increase of EZH2 enrichment at the Exon 3 locus of Tbx1^+/− embryos. Each dot represents an independent experiment using one embryo. (C) Gene expression measured by quantitative real time PCR of cDNA from whole E8.5 embryos (Wnt5a is a transcriptional target of TBX1). We found no change of expression of the two genes in Tbx1^+/−;Ezh2^+/− embryos compared to Tbx1^+/− embryos. Each dot represents a single embryo. Data are mean±s.e.m. Figures above the blue segments indicate P values, calculated by a paired two-tailed Student's t-test using GraphPad software.