Male and female rats received HMWH (1 μg/5 μL) or vehicle (5 μL) intradermally, on the dorsum of the hind paw, 10 min after LMWH (1 μg/5 μL) was injected in the same site. Mechanical nociceptive threshold was evaluated over the 30 min after LMWH administration.
A.
Male: HMWH significantly attenuated LMWH-induced hyperalgesia in males (F(1,10)=33.54, *p=0.0103, when the LMWH-induced hyperalgesia is compared between HMWH- and vehicle-treated groups 10 min after intradermal LMWH; **p=0.0040, when the LMWH-induced hyperalgesia is compared between HMWH- and vehicle-treated groups 15 and 30 min after intradermal LMWH; ***p=0.0001, when the LMWH-induced hyperalgesia is compared between HMWH- and vehicle-treated groups 20 min after intradermal LMWH; two-way repeated-measures ANOVA followed by Holm-Šídák multiple comparison test). n=6 paws per group.
B.
Female: HMWH did not significantly attenuate LMWH-induced hyperalgesia in females (F(1,11)=0.6400, p=0.440, when the LMWH-induced hyperalgesia is compared between HMWH- and vehicle-treated groups after intradermal LMWH; two-way repeated-measures ANOVA followed by Holm-Šídák multiple comparison test). n=6 paws per group.